Together with introduction to clinical practice of modern x-ray, ultrasound and radiological equipment, and also new diagnostic method – threedimensional (3D) virtual NLS-graphy by metatron hunter 4025, we now can carry out combined examination of female patients in order to diagnose breast diseases.

    SEA makes possible to identify morphological character of neoplasm by its spectral similarity to etalon processes, evaluate metastatic invasion of lymph nodes, identify presence and character genetic aberrations in tumoral subclones, which, finally, will identify extent of surgical intervention. Application of three-dimensional NLS

-graphy is most reasonable in the following cases:

– to detect primary nidus at metastatic invasion of axillary lymph nodes;

– to specify extent of tumor spreading;

– for differential diagnostics of cicatrical changes and malignant neoplasms;

– for diagnosing of lesser forms of breast cancer, especially when there are vastly developed glandular tissue and fibrous-cystic mastopathy;

– at multicentric and infiltrating forms of cancer;

– when patient have large breasts and research by other methods of radiodiagnostis is not possible;

– for research of sharp painful glands at fibrouscystic mastopathy, when palpation and compression of mammary gland is not possible;

– for planning of organs-preserving operations.

    Information value of SEA at NLS-ultramicroscopy is conditioned by potential to detect such specific symptoms of tumoral affection as carcinogenesis at gene level. At malignant tumors in chromosome apparatus of mammary glands cells one may detect loci with specific aberrations, related to tumor development. Aberrations number increasing reliably proves presence of malignant neoplasm and degree of tumor malignisation.

    Malignant tumors were visualized at macroscans as nidi of hyperchromic signal (5 – 6 points according to Fleindler’s scale) of various form and size, often with uneven, indistinct outlines.

    Information value of mammary glands NLS-examination was 42% – 67% higher than when we used x-ray mammography and ultrasound examination.

    Metastasis was detected quite accurately if in lymph node was hyperchromic area (6 points), which at SEA had high spectral similarity to «Metastatic tumoral subclon» etalon (D<0.425).

    Application of three-dimensional NLS-graphy opens up great possibilities in diagnostics of both primary and poorly diagnosed forms of breast cancer due to its low price, non-invasive character, high resolution and accuracy (especially when used together with SEA), absence of radio stress and usability.

    Therefore rational sequence of diagnostic methods application in combined diagnostics with obligatory morphological component allows carrying out of accurate diagnostics of mammary glands various pathological conditions with the least costs and time.