Major problems when performing NLS-research with biophilia nls are faced when diagnosing the invasion into the submucous layer or when diagnosing the onset of invasion into the muscular layer. Spectral-entropy analysis often allows solving of such problems.

Tumors (especially endophytic ones) which affect major part of bladder wall may cause its sharp size reduction (microcystis). Small bladder capacity in such patients and also in patients who were surgically treated (often repeated due to repeated relapses) may complicate the detection of tumor’s invasion depth.

Urinary bladder tumors (more often multifocal) in combination with intravesical clots may be complicated for interpretation. Intravesical clots accompanied by macrohaematuria sometimes fill the whole bladder cavity. In these cases it is always possible to differentiate the tissue of the tumor nodule from clots using spectral-entropy analysis.

Diagnostic difficulties appear when detecting the depth of tumor invasion if the nodule is located in urinary triangle (Lieutaud body) especially when it overlaps on the contour of prostate gland. Image unsharpness of wall layers may be caused by multiple surgical interferences in case of bladder tumor relapses and also intravesical immune- or chemotherapeutical treatment and layering of urinary salts.

The sensitivity of 3D-NLS in detection of tumor invasion depth in such patients may be decreased.