Diagnostic value of NLS-graphy in respect to knee joint pathology is extremely high for non-invasive method. It is proven that at acute trauma of knee joint NLS-graphy reliably decrease time needed for diagnosing, improves quality of life within the first 6 weeks after a trauma, decreases total expenses. Initial signs of degenerative changes of arthrodial cartilage in form of superficial separation of fibers are visualized rather good, because to do so a very high resolution – down to 30 microns is required and “Biophilia NLS” system has such resolution characteristics. Evaluation of anterior cruciate ligament condition, which was quite difficult using systems of the previous generation (Biophilia Tracker), can be successfully fulfilled using systems with 3D-visualization feature.

We tried to improve diagnostic possibilities of NLS-scanning by wide application of three-dimensional modes of morphological structures visualization, such as 4D tissue, multiscanning in three mutually perpendicular planes, DeepVision, FastVision and ultramicroscanning with spectral-entropic analysis (SEA).

For proper ultramicroscanning a resolution of NLS-systems must not exceed 30 microns, for cytological studies – less than 10 microns, so the only real way to achieve this is to use algorithms of three-dimensional visualization, such as MIP (Maximum Intensity Projection, MPR (Multi-Planar Reconstruction) and DVR (Direct Volume Rendering).